Comparative metabolic conversion of aflatoxin B1 to M1 and Q1 by monkey, rat and chicken liver |
| |
Authors: | M S Masri A N Booth D P Hsieh |
| |
Affiliation: | Western Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Berkeley, California 94710, USA;Department of Environmental Toxicology, University of California, Davis, California 95616, USA |
| |
Abstract: | We studied the metabolish of flatoxin B1 by liver microsomal preparations from monkey, rat and chicken. With all these species, both the previously recognized metabolite aflatoxin M1 as well as the newly identified aflatoxin Q1 were produced from the aflatoxin B1 substrate. Aflatoxin Q1 is an isomer of aflatoxin M1 (with the hydroxyl on the carbon β to the carbonyl of the cyclopentenone ring) which we discovered recently in rat and monkey liver incubations with aflatoxin B1. In our incubations we did not detect aflatoxin P1 which has been reported as a major metabolite of aflatoxin B1 in the monkey.In general the conversion to aflatoxin M1 was comparable among the different species (1–3% of the substrate) except in the chicken in which it was lower (0.1–0.3%). Also the conversion to Q1 was comparable to or slightly higher than the conversion to M1 with rat and chicken liver but the conversion to Q1 with the monkey liver was outstandingly high, accounting for 19–52% of the substrate in three species of monkeys tested. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|