INSTABILITY OF MESSENGER AND RIBOSOMAL RNA IN A GLUE-PROTEIN MUTANT OF Bombyx mori |
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| Authors: | SEIJI KAMIJO YUTAKA KAWAGUCHI HIROSHI FUJII BUNGO SAKAGUCHI HIROSHI DOIRA SATORU KUHARA YOICHI ASO KATSUMI KOGA KATSUYA HAYASHI KUNITADA SHIMOTOHNO |
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| Affiliation: | Laboratory of Sericulture, Faculty of Agriculture, Kyushu University, Fukuoka 812.;Institute of Silkworm Genetics, Faculty of Agriculture, Kyushu University, Fukuoka 812.;Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University, Fukuoka 812.;National Institute of Genetics, Mishima 411, Japan |
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| Abstract: | The mucous glands of Bombyx pupae secrete glue proteins which attach deposited eggs to the mounting sheet. A mutant of a dominant gene, named no glue (Ng) , produces nonadhesive eggs which have a low capacity for glue-protein synthesis. In the present study it was shown that the mucous glands of Ng silkworms showed rapid degradation of mRNA as well as rRNA during development; this may cause the low capacity for glue-protein synthesis in the mutant organ. In contrast, the mucous glands of normal silkworms showed a significant increase in content of RNA's until the maximum rate of glue-protein synthesis was achieved. The degradation of RNA in the Ng mucous gland was inhibited by actinomycin D injected into the body fluid. Thus it is supposed that the Ng gene codes for a presumptive controller RNA, which would be the mediator of RNA instability in the mucous glands of Ng pupae. |
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