Tempol protects the gallbladder against ischemia/reperfusion |
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Authors: | Pedro J Gomez-Pinilla Pedro J Camello Jesus A F Tresguerres María José Pozo |
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Institution: | 1.Department of Physiology, Nursing School,University of Extremadura,Caceres,Spain;2.Department of Physiology, Medicine School,University Complutense of Madrid,Madrid,Spain;3.Department of Physiology, Nursing School,Avda Universidad s/n,Cáceres,Spain |
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Abstract: | Impairment in gallbladder emptying, increase in residual volume, and reduced smooth muscle contractility are hallmarks of
acute acalculous cholecystitis and seem to be related to ischemia/reperfusion (I/R). This study was designed to determine
the effects of tempol, a general antioxidant, on I/R-induced changes in gallbladder contractile capacity, the mechanisms involved
in the contractile process, and the level of inflammatory mediators. Experimental gallbladder I/R was induced in male guinea
pigs by common bile duct ligation for 2 days, then a deligation of the duct was performed and after 2 days the animals were
sacrificed. A group of animals was treated with tempol, administered in the drinking water at 1 mmol/l for 10 days prior the
bile duct ligation and until animal sacrifice. Isometric tension recordings showed that KCl and cholecystokinin-induced contractions
were impaired by I/R, which correlated with decreased F-actin content and detrimental effects on Ca2+ influx. In addition, I/R depolarized mitochondrial membrane potential, as indicated by the reduction of the heterogeneity
of the rhodamine123 fluorescence signal, and increased the expression of NF-κB, COX-2, and iNOS. Tempol treatment improved
contractility via normalization of Ca2+ handling and improvement of F-actin content. Moreover, the antioxidant ameliorated mitochondrial polarity and normalized
the expression levels of the inflammatory mediators. These results show that antioxidant treatment protects the gallbladder
from I/R, indicating the potential therapeutic benefits of tempol in I/R injury. |
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