Isolation and characterization of 16 microsatellite loci in an endangered fish Ussuri cisco, <Emphasis Type="Italic">Coregonus usssruensis</Emphasis> |
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| Authors: | Dongmei Yu Bo Ma Yanhong Sun Jianjun Peng |
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| Institution: | (1) South China Institute of Endangered Animals, No. 105 Xin Gang Xi Road, 510260 Guangzhou, China;(2) Heilongjiang River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin, 150070, China;(3) School of Life Science, Huazhong Normal University, 430079 Wuhan, China |
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| Abstract: | Sixteen polymorphic microsatellite loci were isolated from an enriched genomic library of Ussuri cisco, Coregonus usssruensi. The polymorphism of these loci was tested on a population of 30 individuals captured from Heilongjiang River. The number
of observed alleles per locus ranged from 3 to 18. The expected and observed heterozygosity values ranged from 0.126 to 0.919
and 0.133 to 0.833, respectively. Five loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction.
No significant linkage disequilibrium was detected. These polymorphic markers will be used to assess population structure
in Ussuri cisco. |
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