An automated fluorescence method for determination of K+-dependent phosphatase |
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| Authors: | E O Titus W W Holland E A Brown W M Hart |
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| Affiliation: | Laboratory of Chemical Pharmacology, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014 USA |
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| Abstract: | An automated fluorescence method for the determination of K+-dependent umbelliferone phosphatase activity is described. This activity is associated with a (Na+ + K+)-dependent ATPase in brain and kidney microsomal preparations. The ratio of phosphatase activity/ATPase activity is greater in kindney. |
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