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An automated fluorescence method for determination of K+-dependent phosphatase
Authors:E O Titus  W W Holland  E A Brown  W M Hart
Affiliation:Laboratory of Chemical Pharmacology, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014 USA
Abstract:An automated fluorescence method for the determination of K+-dependent umbelliferone phosphatase activity is described. This activity is associated with a (Na+ + K+)-dependent ATPase in brain and kidney microsomal preparations. The ratio of phosphatase activity/ATPase activity is greater in kindney.
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