丛枝菌根真菌对红花根围微生物多样性特征的影响

目的]研究丛枝菌根(Arbuscular mycorrhiza,AM)真菌对红花根围微生物多样性特征的影响.方法]对红花接种Glomus mosseae、G.intraradices和混合菌(G.mosseae、G.intraradices、G.cladoideum、G.microagregatum、G.caledonium、G.etunicatum)3种AM真菌,采用传统的平板稀释涂布法对红花根围细菌、真菌、放线菌进行分离、培养、计数和鉴定,并结合单链构象多态性(Single stand conformational polymorphism,SSCP)分析技术对红花根围微生物多样性进行分析.结果]3处理与对照组红花根围微生物总量表现为G.mosseae＞G.intraradices＞混合菌＞对照,且接种处理红花根围微生物总量显著高于对照.对照组红花各生长期微生物总量均为0-5 cm＞5 cm-10 cm＞10 cm-20 cm,接种处理红花生长至种子成熟期时不同土层微生物数量发生变化,微生物总量为5 cm-10 cm＞0-5 cm＞10 cm-20 cm.聚类分析发现微生物出现接种红花类群与不接种红花类群两大分类类群.结论]AM真菌从时间和空间上影响了红花根围微生物的多样性特征,对红花农业生产和分析生态系统中微生物之间的相互作用具有重要的理论和实践价值.

How arbuscular mycorrhiza fungi affect on rhizosphere microbe diversity of Carthamus tinctorius L. in Xinjiang

Objective] Demonstrate the effects of Arbuscular mycorrhiza (AM) fungi on Carthamus tinctorius L. rhizosphere microbe diversity. Methods] Dilution cultural and PCR-single stand conformational polymorphism (SSCP) were used for determing rhizosphere microbe exist in the ambient of inoculated AM fungi plants Carthamus tinctorius L. or named safflower as well. Target safflowers were inoculated with Glomus mosseae, G. intraradices and mixed group (G. mosseae, G. intraradices, G. cladoideum, G. microagregatum, G. caledonium and G. etunicatum), respectively. Results] Total number of rhizosphere microbe around treated safflower shows an order of G. mosseae>G. intraradices>mix. Compared with control group, inoculated group has larger quantity (p<0.05) of total microbe number according the order of 0?5 cm>5 cm?10 cm>10 cm?20 cm in most of its growth stages. Inoculated safflower, however, showed differ microbial quantity along soil vertical profile as 5 cm?10 cm> 0??5 cm>10 cm?20 cm. Clustering analysis confirmed exist as inoculation and non-inoculation plant microbial groups. Conclusion] AM fungi have an affected on the variation pattern of rhizosphere microbe diversity of safflower by temporally and spatially varieties. This patterns not only can help us easy understanding the significance function of microbe exist in terrestrial ecosystem, could also support as a guidance in appropriate cultivation strategy on agricultural production the near future.