虫压胁迫对抗虫转基因水稻生长发育及产量性状的影响

在大田条件下，以转基因抗虫水稻Bt63、R1和R2及非转基因水稻汕优63(对照)为材料，设置高、低两种虫压环境条件，研究虫压胁迫对转Bt抗虫基因水稻生长发育及产量相关性状的影响.结果表明： 抗虫水稻在虫压胁迫条件下可充分体现出外源基因的抗性特点.在高虫压条件下，3种转Bt基因水稻受螟虫危害程度远低于对照植株，株高、分蘖数、地上部鲜质量、穗长、穗质量、单株穗数、单株实粒数、实粒质量、结实率、千粒重等生长发育和产量指标均高于对照，但仅株高、分蘖数和穗长3个指标与对照有显著差异.因此，抗虫外源Bt基因的引入对水稻结实性不会产生负面效应，高虫压胁迫条件对抗虫转基因水稻产量的影响较小.

Effects of herbivorous insect stress on the growth and yield-related traits of insect-resistant transgenic rice

A field experiment was conducted to test the effects of high and low herbivorous insect stresses on the growth, development and yield related traits of insect resistant transgenic rice. Three Bt transgenic rice Bt63, R1 and R2 were selected as the test materials, and non transgenic rice Shanyou63 was taken as the control. The differences in the vegetative growth, seed setting, and stem borer resistance between transgenic and non transgenic rice were compared. Under herbivorous insect stress, the transgenic rice fully displayed the stem borer resistance of exogenous gene. Under the high stress, the stem borer damaged degree of the three transgenic rice lines was much lower than that of the control. The plant height, tillers per plant, aboveground fresh mass, panicle length, panicle fresh mass, productive panicles per plant, filled grains per plant, grain mass per plant, seed setting rate, and 1000 grain mass of the three transgenic rice lines excelled the control, but had no significant differences except plant height, tillers per plant, and panicle length. Therefore, introducing exogenous Bt gene into rice had no negative effect on rice seed setting, and high herbivorous insect stress had less impacts on transgenic rice yield.