Application of PCR, rep-PCR and RAPD techniques for typing ofLactococcus lactis strains |
| |
Authors: | J Prodělalová A Španová B Rittich |
| |
Institution: | Department of Microbiology, Faculty of Science, Masaryk University, 602 00 Brno, Czechia. prodelalova@vri.cz |
| |
Abstract: | A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic identification of Lactococcus lactis strains by RAPD; rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterization. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|