Heat shock and cold shock in Deinococcus radiodurans |
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| Authors: | Alessandro Airo Suzanne L Chan Zak Martinez Manu O Platt Jonathan D Trent |
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| Institution: | (1) Department of Geological & Environmental Sciences, Stanford University, Stanford, CA;(2) SETI Institute, Mountain View, CA;(3) Chemistry Department, University of California Santa Cruz, Santa Cruz, CA;(4) Department of Biomedical Engineering, Georgia Tech/Emory University, Atlanta, GA;(5) Astrobiology Technology Branch, NASA Ames Research Center, Moffett Field, CA |
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| Abstract: | On the basis of acquired thermotolerance and cryotolerance, the optimal heat shock and cold shock temperatures have been determined
for Deinococcus radiodurans. A heat shock at 42°C maximized survival at the lethal temperature of 52°C and a cold shock at 20°C maximized survival after
repeated freeze-thawing. Enhanced survival from heat shock was found to be strongly dependent on growth stage, with its greatest
effect shortly after phase. Increased synthesis of a total of 67 proteins during heat shock and 42 proteins during cold shock
were observed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and autoradiography. Eight of the most highly
induced heat shock proteins shown by 2D PAGE were identified by MALDI-MS as Hsp20, GroEL, DnaK, SodA, Csp, Protease I and
two proteins of unknown function. |
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| Keywords: | Thermotolerance cryotolerance HSP CSP extremophile stress chaperone |
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