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A plasmid cloning vector for Kpnl-cleaved DNA
Authors:K Beckingham
Institution:Department of Biochemistry, Rice University, Houston, Texas 77001 U.S.A.
Abstract:A plasmid cloning vector containing a single site for KpnI has been generated by insertion of a 3.5-kb EcoRI/HindIII fragment of pCR1 into the EcoRI/HindIII sites of pBR322. KpnI cleavage yields 3′ rather than 5′ “sticky ends” which allows reconstitution of the recognition site after cloning by a homopolymer joining procedure. This is an advantage shared with only one or two other commercially available restriction enzymes.
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