Identifying novel proteins recognizing histone modifications using peptide pull-down assay |
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Authors: | Wysocka Joanna |
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Institution: | Department of Molecular Pharmacology and Developmental Biology, Stanford University, 269 Campus Drive, Stanford, CA 94305, USA. wysocka@stanford.edu |
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Abstract: | Post-translational modifications of histones have been correlated with virtually all chromatin-templated processes, including gene expression regulation, DNA replication, mitosis and meiosis, and DNA repair. In order to better understand the mechanistic basis by which histone modifications participate in the control of cellular processes, it is essential to identify and characterize downstream effector proteins, or "readers", that are responsible for recognizing different marks and translating them into specific biological outcomes. Ideally, identification of potential histone-binding effectors should occur in an unbiased fashion. Although in the recent years much progress has been made in identifying readers of histone modifications, in particular methylation, recognition of the majority of known histone marks is still poorly understood. Here I describe a simple and unbiased biochemical pull-down assay that allows for the identification of novel histone effector proteins and utilizes biotinylated histone peptides modified at various residues. I provide detailed protocols and suggestions for troubleshooting. |
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Keywords: | Histone modifications Peptide pull-down Chromatin Effector protein Histone methylation Histone acetylation Histone phosphorylation Biotinylated peptide |
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