Essential role of SRC suppressed C kinase substrates in Schwann cells adhesion, spreading and migration |
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Authors: | Yan Meijuan Cheng Chun Jiang Jing Liu Yonghua Gao Ying Guo Zhiqin Liu Haiou Shen Aiguo |
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Institution: | (1) Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China |
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Abstract: | Integrin-mediated substrate adhesion of endothelial cells leads to dynamic rearrangement of the actin cytoskeleton. Protein
kinase C (PKC) stimulates reorganization of microfilaments and adhesion, while the responses of Schwann cells during adhesion
and migration are unknown, so we examined the expression changes of SSeCKS and F-actin in Schwann cells after exposure to
fibronectin. Src (sarcoma) suppressed C kinase substrate (SSeCKS) is a PKC substrate that may play an important role in regulating
actin cytoskeleton. We found that SSeCKS was localized to focal adhesion sites soon after Schwann cells adhesion and that
SSeCKS increased during the process of cell spreading. Using small interfering RNAs specific to SSeCKS, we showed that Schwann
cells in which SSeCKS expression was inhibited reduced cellular adhesion, spreading and promoted cellular migration on fibronectin
through reorganization of actin stress fibers and blocking formation of focal adhesions. These results demonstrated SSeCKS
modulate Schwann cells adhesion, spreading and migration by reorganization of the actin cytoskeleton. |
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Keywords: | Rat Schwann cell Src suppressed C kinase substrates Cell adhesion Small interfering RNA |
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