Optimization of process variables in castor oil hydrolysis by Candida rugosa lipase with buffer as dispersion medium |
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Authors: | Debajyoti Goswami Jayanta Kumar Basu Sirshendu De |
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Institution: | (1) Department of Chemical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India |
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Abstract: | In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process:
speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions
are 1,100 rpm, pH 6.5, temperature 35°C, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions,
the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after
the first recycle. |
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Keywords: | castor oil candida rugosa lipase hydrolysis ricinoleic acid |
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