Generation of Marburg virus-like particles by co-expression of glycoprotein and matrix protein |
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Authors: | Swenson Dana L Warfield Kelly L Kuehl Kathleen Larsen Thomas Hevey Michael C Schmaljohn Alan Bavari Sina Aman M Javad |
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Institution: | U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD 21702, USA. |
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Abstract: | Marburg virus (MARV), the causative agent of a severe hemorrhagic fever, has a characteristic filamentous morphology. Here we report that co-expression of MARV glycoprotein and matrix protein (VP40) in mammalian cells leads to spontaneous budding of filamentous particles strikingly similar to wild-type MARV. In addition, these particles elicit an immune response in BALB/c mice. The generation of non-replicating Marburg virus-like particles (VLPs) should significantly facilitate the research on molecular mechanisms of MARV assembly and release. Furthermore, VLPs may be an excellent vaccine candidate against Marburg infection. |
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