Maintenance of plasmids in HU and 1HF mutants of Escherichia coli |
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Authors: | Tern Ogura Hironori Niki Yasunobu Kano Fumio Imamoto Sota Hiraga |
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Affiliation: | (1) Department of Molecular Genetics, Institute for Medical Genetics, Kumamoto University Medical School, 862 Kumamoto, Japan;(2) Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, 1 Shichono-cho, Misasagi, 607 Yamashina-ku, Kyoto, Japan |
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Abstract: | Summary Complementation and sequencing analyses revealed that the hopD mutants, which could not support stable maintenance of mini-F plasmids (Niki et al. 1988), had mutations in the hupB gene, and that the hopD410 mutation was an ochre mutation at the 5th Gln position of HU-1. Maintenance and stability of various plasmids, mini-P1 plasmids, mini-F plasmids, and oriC plasmids, were studied in the hupA and hupB mutants (HU mutants), and himA and hip mutants (IHF mutants). Mini-P1 plasmids and mini-F plasmids could not be introduced into the hupA-hupB double deletion mutant. Replication of mini-F plasmids was partially inhibited in the hupB mutants, including the hupB and hopD(hupB) mutants, whereas replication of oriC plasmids was not significantly affected even in the hupA-hupB double deletion mutant. The mini-P1 plasmid was slightly unstable in the himA-hip mutant, whereas the mini-F plasmid was stable. |
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Keywords: | Mini-P1 plasmid Mini-F plasmid Replication hupA-hupB mutant himA-hip mutant |
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