纳米金生物条形码技术检测痕量二噁英类化合物

建立一种基于纳米金生物条形码技术的二噁英快速筛检方法.利用二噁英诱 导激活的芳香烃受体复合物，特异识别以纳米金为报告基团的二噁英反应探针，该 探针被释放后进行条形码放大，通过纳米金银染技术增强识别信号，并记录其吸光度值，从 而可以简单灵敏地快速筛检二噁英类化合物.在一定的反应时间和浓度范围内（10^-14～10^-10mol/L），溶液的吸光度值与2，3，7，8 四氯二苯并二噁英（2，3，7，8-tetrachlorodibenzo-p-dioxin，TCDD）浓度之间呈正相关 ，方法检测限为0.01 pmol/L，变异系数为5%～8%.用纳米金生物条形码（Nano Barcod e，nanoparticle based bio barcode）方法和现有的生物分析方法（CALUX，chemical activated luciferase gene expression）分别测定TCDD标准品，并绘制剂量效应曲线.结 果表明，本方法灵敏度高，线性范围宽，重复性好.本研究纳米金生物条形码方法的检测灵 敏度高于CALUX将近5倍(EC₅₀分别为 4×10^-12 mol/L 和 2×10^{- 11} mol /L)，检测限较CALUX降低了10倍（检测限分别为1×10^-14 mol/L 和1×10 ^-13 mol/L），变异系数分别为5%～8%和15%～30%.

Nanoparticle based Bio barcode Method for Trace Analysis of Dioxin-like Chemicals

We have established a nanoparticle based bio barcode method for the prescreening of dioxins.The formation of AhR Arnt complex triggered by dioxin resulted in a high affinity to DRE.The DRE probes were completely released as bio-barcodes and amplified by nanoparticle based silver enhancement，then the absorbance of TCDD（2，3，7，8-tetrachlorodibenzo-p-dioxin）were recorded as quantitative signals.The dose dependency of the assay range between 10^-14mol/Lto 10^-10mol/L for soluable products.The TCDD detection limit was 0.01 pmol/Lwith a coefficient of variation as 5%-8%Compared with CALUX（chemical-activated luciferase gene expression） bioassay，the sensitivity of our Nano-Barcode method was approximately 5 times higher with EC₅₀of 4×10^-12mol/L，and the detection limit was 10 times lower as 1×10^-14 mol/L .In addition，our method exhibited a better reproducibility of CV as 5%-8% compared to 15%-30% of CALUX.