Separation of epithelial cells from suspensions of cells from the hamster parotid gland in an isokinetic density gradient of Ficoll in tissue culture medium. |
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Authors: | D W Aspray A Pitts T G Pretlow |
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Affiliation: | 1. Department of Pathology, University of Alabama Medical Center, Birmingham, Alabama 35294 USA;2. Department of Engineering Biophysics, University of Alabama Medical Center, Birmingham, Alabama 35294 USA |
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Abstract: | Epithelial cells were separated from suspensions of hamster parotid cells by velocity sedimentation in an isokinetic gradient and by isopycnic sedimentation. Epithelial cells were 48.1 ± 18.0% of the cells in the starting sample suspensions of cells from the disaggregated hamster parotid glands. The purest gradient fractions following velocity sedimentation in a previously described isokinetic gradient contained 98.8 ± 1.8% epithelial cells. The purest fractions obtained from isopycnic sedimentation contained 99.9 ± 0.2% epithelial cells. Purification of parotid epithelial cells by velocity sedimentation in the isokinetic gradient seems preferable to purification using isopycnic centrifugation because a larger proportion of the epithelial cells are obtained in the zone of the gradient which contains highly purified epithelial cells and because velocity sedimentation requires lower centrifugal forces for a shorter period of time. |
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Keywords: | Recipient of NIH Research Career Development Award (No. K4-CA-70584-01) to whom requests for reprints should be sent. |
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