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Type A and B gaba receptors mediate inhibition of acetylcholine release from cholinergic nerve terminals in the superior cervical ganglion of rat |
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| Authors: | Z Farkas P K sa V J Balcar F Jo and J R Wolff |
| Institution: | * Central Research Laboratory, Medical University, Szeged, Hungary ? Developmental Neurobiology Unit, Department of Anatomy, University of Gottingen, Göttingen, F.R.G. ? Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary |
| Abstract: | The effects of γ-amino-n-butyric acid (GABA), (+)bicuculline, isoguvacine and 3-(4-chlorophenyl)-4-aminobutyrate (±)baclofen] on the K-induced release of 3H]acetylcholine (ACh) were studied in the superior cervical ganglia of the rat in vitro. GABA and isoguvacine inhibited 3H]ACh release and these inhibitions were reversible by (+)bicuculline. Furthermore, the release of 3H]ACh was also inhibited by (±)baclofen. In receptor-binding studies, binding of 3H]GABA to membrane preparations from the superior cervical ganglia was inhibited by both (±)baclofen and (+)bicuculline. It is concluded that the inhibitory effect of GABA on the release of ACh can be mediated by GABAA(bicuculline-sensitive) and by GABAB (baclofen-activated) receptors. Our findings are compatible with the existence of a non-synaptic GABAergic inhibitory system involving GABAA and GABAB receptors on cholinergic nerve terminals in the superior cervical ganglion of rat. |
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