| 定量蛋白质组学分析方法及应用 |
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| 引用本文： | 应天翼,王恒樑,黄留玉,黄翠芬. 定量蛋白质组学分析方法及应用[J]. 生物技术通讯, 2005, 16(1): 90-92 |
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| 作者姓名： | 应天翼 王恒樑 黄留玉 黄翠芬 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 摘 要： | 蛋白质组学经历了近10年的发展，现在已经初具规模。但是由于它是动态地观察生物体不断变化的所有蛋白质，所以技术难度非常之大。为使研究简化并更具针对性，人们着重进行比较蛋白质组学的研究。为了具体量化这些蛋白质的变化产生了定量蛋白质组学，近几年各种标记技术的进步使得该学科得以迅猛发展。
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| 关 键 词： | 定量蛋白质组学 同位素标记 双向电泳 蛋白质组学 特定氨基酸残基 荧光染料标记 |
| 文章编号： | 1009-0002(2005)01-0090-03 |
| 修稿时间： | 2004-06-21 |
Analysis Methods and Applications of Quantitative Proteomics |
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| YING Tian-yi,WANG Heng-liang,HUANG Liu-yu,HUANG Cui-fenInstitute of Biotechnology,Academy of Military Medical Sciences,Beijing ,China. Analysis Methods and Applications of Quantitative Proteomics[J]. Letters in Biotechnology, 2005, 16(1): 90-92 |
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| Authors: | YING Tian-yi WANG Heng-liang HUANG Liu-yu HUANG Cui-fenInstitute of Biotechnology Academy of Military Medical Sciences Beijing China |
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| Affiliation: | YING Tian-yi,WANG Heng-liang,HUANG Liu-yu,HUANG Cui-fenInstitute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China |
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| Abstract: | Although proteomics has progressed enormously in the past decade, many questions remain. Monitoring protein levels is crucial and difficult to obtaining a complete picture of various biological processes. In order to simplify the research, scientists focus on the comparative proteomics study. So quantitative proteome analysis is increasingly being used as a method to study steady-state and perturbation-induced changes in protein profiles. |
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| Keywords: | quantitative proteomics isotope labeling two-dimensional gel electrophoresis |
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