Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. |
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| Authors: | M G Marinus N R Morris |
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| Affiliation: | Department of Pharmacology, College of Medicine and Dentistry of New Jersey, Piscataway, N.J. 08854 U.S.A. |
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| Abstract: | The dam-3 mutation results in a five-fold reduction in the number of 6-methyl-adenine (6-meA) residues in the DNA of E. coli K12 or phage lambda. The DNA of phage fd appears to be devoid of 6-meA when propagated on dam-3 bacteria. The phenotypic differences between dam-3 and dam+ bacteria include: (i) increased free phage in lysogenic dam-3 cultures, (2) increased sensitivity to methyl methanesulfonate (MMS), (3) inviability of dam-3 lex-I strains, (4) lower molecular weight of DNA in dam-3 bacteria in the absence of DNA ligase and (5) increased rate of DNA degradation in dam-3 recA strains. |
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| Keywords: | endonuclease I 6-meA 5-mec 5-methylcytosine MMS methyl methanesulfonate |
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