Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley (Hordeum vulgare L.)

Segregation distortion is a common phenomenon among anther-culture-derived plants and it has been suggested that the distorted areas may contain genes affecting survival in anther-culture. Segregation of 111 markers was checked in an androgenetic barley (Hordeum vulgare L.) progeny and a linkage map was constructed. Thirty one progeny lines were tested for their anther-culture ability and associations between molecular markers and anther-culture traits were established. Two regions on chromosomes 2H and 4H were associated with anther response, three on 2H (two areas) and 3H with plant regeneration rate, and one on 4H with spontaneous diploidization. The chromosomal regions controlling anther-culture response and the regions where distorted segregation was found were not always the same. Received: 19 February 1999 / Accepted: 30. April 1999