Screening of thermotolerant yeasts as producers of superoxide dismutase |
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Authors: | TS Nedeva VA Savov AV Kujumdzieva-Savova ER Davidov |
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Institution: | Department of Microbiology, Faculty of Biology, University of Sofia, Sofia, Bulgaria; VNII Synthesbelok, Moscow, Russia |
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Abstract: | Abstract A screening procedure for highly thermostable yeast superoxide dismutase was developed. Growth yields at various temperatures were estimated for ten mesophilic and thermotolerant strains, belonging to the genera Saccharomyces, Kluyveromyces and Pichia . Higher yields at 45°C were obtained for K. lactis 90-3 and 90-4. A correlation between the ability to grow at higher temperature and the thermostability of the superoxide dismutase enzyme synthesized was observed. A comparison of the operational stability of the superoxide dismutase of all tested strains suggests that the enzyme of K. lactis strains was more thermostable than that of the other tested microorganisms. |
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Keywords: | Yeast Superoxide dismutase Thermostability Operational stability |
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