Reliable and rapid discrimination of congeneric species by mtDNA SNP analysis by multiplex PCR: application on three Trachurus and two Mullus fish species as model cases |
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| Authors: | Apostolos P Apostolidis Andreas Georgiadis Nikoletta Karaiskou Raphael Sandaltzopoulos |
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| Institution: | (1) Department of Animal Production, Faculty of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece;(2) Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece;(3) Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, 68100, Greece |
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| Abstract: | We present a reliable, time-saving, and cost-effective multiplex PCR assay for discriminating congeneric species. Three fish
species of the genus Trachurus and two of the genus Mullus served as model cases. Our multiplex PCR method interrogates species-specific diagnostic mitochondrial single nucleotide
polymorphisms (mtSNPs). We selected two sets of mtSNPs that are organized in two multiplex systems for the Trachurus and the Mullus species, respectively. In both systems, all individuals tested could be safely assigned to one of the three Trachurus or two Mullus species. This novel SNP typing system offers a convenient and robust DNA profiling system suitable for large-scale identification
of commercial fish species, for species with cryptic larvae or during juvenile stages, as well as for wildlife forensics.
Handling editor: Christian Sturmbauer |
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| Keywords: | Mitochondrial DNA Single Nucleotide Polymorphisms (SNPs) Multiplex PCR Species discrimination Trachurus Mullus |
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