Genetic manipulation of microspores and microspore-derived embryos |
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Authors: | Bin Huang |
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Institution: | (1) Allelix Crop Technologies, (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Drive, L4V 1P1 Mississauga, Ontario, Canada |
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Abstract: | Summary Recent advances in plant cell and molecular biology have furthered the genetic manipulation of many plant species and advanced
the options for crop improvement. Among the many targets for genetic manipulation, microspores offer several unique advantages:
they are haploid, single-celled, and highly synchronized. In many plant species microspores develop into haploid embryos,
and eventually haploid and doubled haploid plants, after in vitro anther or microspore culture. This induced in vitro developmental
pathway of microspores, termed microspore embryogenesis, can be used to recover individual homozygous plants from microspores
and microspore-derived embryos after genetic manipulation such as mutagenesis and gene transfer. The highly efficient microspore
embryogenesis system inBrassica napus has been used successfully to obtain various mutants after microspore mutagenesis, and to achieve gene transfer mediated
byAgrobacterium tumefaciens.
Presented in the Session-in-Depth In Vitro Gametophyte Biology at the 1991 World Congress on Cell and Tissue Culture held
in Anaheim, California, June 16–20, 1991. |
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Keywords: | anther culture haploid embryos doubled haploid plants microspore embryogenesis microspore mutagenesis gene transfer Brassica napus |
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