Reversible skeletal neuromuscular paralysis induced by different lysophospholipids |
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| Authors: | Caccin Paola Rigoni Michela Bisceglie Alessandra Rossetto Ornella Montecucco Cesare |
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| Affiliation: | Dipartimento di Scienze Biomediche, Istituto C.N.R. Neuroscienze, Università di Padova, Viale G. Colombo n. 3, 35121 Padova, Italy. |
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| Abstract: | Lysophosphatidylcholine rapidly paralyses the neuromuscular junction (NMJ), similarly to snake phospholipase A2 neurotoxins, implicating a lipid hemifusion-pore transition in neuroexocytosis. The mode and kinetics of NMJ paralysis of different lysophospholipids (lysoPLs) in high or low [Mg2+] was investigated. The following order of potency was found: lysophosphatidylcholine>lysophosphatidylethanolamine>lysophosphatidic acid>lysophosphatidylserine>lysophosphatidylglycerol. The latter two lysoPLs closely mimic the profile of paralysis caused by the toxins in high [Mg2+]. This paralysis is fully reversed by albumin washing. These findings provide novel insights on the mode of action of snake neurotoxins and qualify lysoPLs as novel agents to study neuroexocytosis. |
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| Keywords: | FA, fatty acids OA, oleic acid SPAN, snake phospholipase A2 presynaptic neurotoxin lysoPC, lysophosphatidylcholine lysoPE, lysophosphatidylethanolamine lysoPS, lysophosphatidylserine lysoPG, lysophosphatidylglycerol lysoPA, lysophosphatidic acid lysoPL, lysophospholipid SVs, synaptic vesicles [Mg2+], magnesium concentration PLA2, phospholipase A2 neuromuscular junction, NMJ |
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