Degradation of 1,4-dichlorobenzene by enriched and constructed bacteria |
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Authors: | Rüdiger H. Oltmanns Hans Georg Rast Walter Reineke |
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Affiliation: | (1) Gesamthochschule Wuppertal, Fachbereich 9, Bergische Universität, Gaußstraße 20, D-5600 Wuppertal 1, Federal Republic of Germany;(2) Bayer AG, D-5090 Leverkusen, Federal Republic of Germany;(3) Present address: Institut für Mikrobiologie, Universität Hohenheim, D-7000 Stuttgart 70, Federal Republic of Germany |
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Abstract: | Summary Three strains, RHO1, R3 and B1, tentatively identified as a Pseudomonas sp., an Alcaligenes sp. and a Pseudomonas sp. which were able to use 1,4-dichlorobenzene as the sole carbon and energy source were isolated from water of the Rhine river and from the sewage plant at Leverkusen-Bürrig. A hybrid strain, WR1313, which uses chlorobenzene as the growth substrate, was obtained by mating the benzene-growing Pseudomonas putida strain F1 with strain B13, a Pseudomonas sp. degrading chlorocatechols. Further selection of this strain for growth on 1,4-dichlorobenzene allowed the isolation of strain WR1323. During growth on 1,4-dichlorobenzene the strains released stoichiometric amounts of chloride. The affinity of the organisms to 1,4-dichlorobenzene was measured with strain R3 showing a Ks value of 1.2 mg/l. Respiration data and enzyme activities in cell extracts as well as the isolation of 3,6-dichlorocatechol from the culture fluid are consistent with the degradation of 1,4-dichlorobenzene via 3,6-dichlorocatechol, 2,5-dichloro-cis,cis-muconate, 2-chloro-4-carboxymethylenebut-2-en-4-olide. |
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