On the occurrence of a high content of cytokinins in rice callus tissues |
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Authors: | Inoue Mitsugu; Maeda Eizo; Yoshida Ryuji; Oritani Takashi |
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Institution: | 1Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University Nagoya
2Toyama Technical College Toyama, Japan |
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Abstract: | Rice callus tissues contained at least three active cytokinincompounds: zeatin, its riboside and N6-(2-isopentenyl) adenosine.These butanol-extractable cytokinins were identified by theirchromatographic mobilities in Sephadex LH-20, paper and gaschromatography. Zeatin, the apparent major cytokinin, was presentat concentrations of 0.7 to 1.0 µg/g fresh tissue and1.3 to 1.7 µg/g fresh tissue in 107 M and 1055M 2,4-D callus, respectively. On the basis of these and earlierresults, the induction and growth of rice callus tissue is discussedin relation to the occurrence of cytokinins in the tissue. (Received December 27, 1978; ) |
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