Chitinolytic properties of Streptomyces lividans |
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Authors: | Ewa Neugebauer Benoit Gamache Claude V. Déry Ryszard Brzezinski |
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Affiliation: | (1) Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 Bld Université, J1K 2R1 Sherbrooke, Québec, Canada |
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Abstract: | Streptomyces lividans TK24, an established host for genetic and molecular studies in actinomycetes, is able to use chitin as sole carbon and nitrogen source. Extracellular chitinase and N-acetyl--d-glucosamidinase (chitobiase) activities were detected in liquid cultures. Chitinase production was inducible by chitin and its low molecular weight derivatives. Low levels of chitinase were also produced in the absence of chitin. Production of extracellular N-acetylglucosaminidase was correlated with the beginning of the stationary phase of growth and was independent of the presence of chitin. Beside highly N-acetylated chitin, supernatants of chitin-induced cultures were able to hydrolyse chitosans with a wide range of degrees of N-acetylation.Abbreviations MS minimal salts - GlcNAc N-acetyl--d-glucosamine - pNP-GlcNAc p-nitrophenyl-2-acetamido-2-deoxy--d-glucopyranoside - d.a. degree of N-acetylation - TLC thin-layer chromatography |
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Keywords: | Streptomyces lividans Chitin Chitosan Chitinase N-Acetylglucosaminidase |
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