Blocking of in vitro translation of Paramecium messenger RNAs is due to messenger RNA primary structure |
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Authors: | E Meyer F Caron B Guiard |
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Institution: | Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique 91190, Gif sur Yvette, France. (Tel.: 6-9077828 ext. 807). (Telex: 691137 F) |
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Abstract: | Paramecium primaurelia mRNAs were translated in vitro in rabbit reticulocyte lysate and the products of translation were analyzed by their size. We show that the large majority of these products are of short but discrete sizes irrespective of the length of the mRNA which directs their synthesis. An illustrative example is given by the translation of mRNA of G surface antigen which directs the synthesis of a 50 kD polypeptide instead of the complete 250 kD protein. Control experiments suggest that the blocking is due to mRNA primary structure. |
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Keywords: | RNA messager antigène de surface messenger RNAs surface antigen |
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