The tricarboxylate carrier from rat liver mitochondria. Purification, reconstitution and kinetic characterization |
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| Authors: | D M Glerum D Claeys W Mertens A Azzi |
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| Institution: | Institut für Biochemie and Molekularbiologie, Universit?t Bern, Switzerland. |
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| Abstract: | The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A Km of 40 microM and a Vmax of 1.56 mumol x min-1 x mg-1 have been determined for the carrier. Cholesterol levels of between 5-10% of total lipid content are shown to cause a decrease in carrier activity. |
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