Production of plantlets of Eleutherococcus sessiliflorus via somatic embryogenesis and successful transfer to soil |
| |
Authors: | Choi YE Ko SK Lee KS Yoon ES |
| |
Institution: | (1) Korea Ginseng Institute, Chung-Ang University, Ansung-shi, Kyunggi-do, South Korea;(2) Panax-Bio Institute, Bugui-meun, Jinan-kun, 567-910, Chollabuk-do, South Korea;(3) Department of Biology, Kongju National University, Kongju, 314-701, South Korea |
| |
Abstract: | Explants of germinating zygotic embryos of Eleutherococcus sessiliflorus,an important medicinal plant, produced somatic embryos directly on Murashige and Skoog (MS) medium with 4.5 M 2,4-D. In addition, embryogenic callus formed at a low frequency (less than 7%) from hypocotyl segments after prolonged culture. High frequency somatic embryogenesis was obtained through cell suspension culture after the cells were transferred to medium lacking 2,4-D. Maturation and germination of embryos was influenced by the sucrose concentration of the medium. At a low concentration of sucrose (1%), maturation and germination of embryos occurred readily. At over 6% sucrose, somatic embryos did not germinate although this could be overcome by GA3 treatment. Cold treatment during acclimatization after transfer to soil enhanced survival. Surviving plantlets produced new sprouts after overwintering in the field. |
| |
Keywords: | plant regeneration Acanthopanax cell culture |
本文献已被 SpringerLink 等数据库收录! |
|