Characterization and mapping of R
Pi-ber
, a novel potato late blight resistance gene from Solanum berthaultii |
| |
Authors: | G M Rauscher C D Smart I Simko M Bonierbale H Mayton A Greenland W E Fry |
| |
Institution: | (1) Department of Plant Pathology, Cornell University, Ithaca, NY, USA;(2) Department of Horticulture, Cornell University, Ithaca, NY, USA;(3) Centro Internacional de la Papa, Lima, Peru;(4) Syngenta Jealott’s Hill, Bracknell, UK;(5) Present address: USDA-ARS, Beltsville, MD, USA;(6) Present address: Department of Plant Pathology, Cornell University, Geneva, NY, USA;(7) Present address: National Institute for Agricultural Botany, Cambridge, UK |
| |
Abstract: | Phytophthora infestans, the causal agent of late blight, threatens potato production worldwide. An important tool in the management of the disease
is the use of resistant varieties. Eleven major resistance genes have been identified and introgressed from Solanum demissum. However, new sources of resistance are continually sought. Here, we report the characterization and refined genetic localization
of a resistance gene previously identified as Rber in a backcross progeny of Solanum tuberosum and Solanum berthaultii. In order to further characterize Rber, we developed a set of P. infestans isolates capable of identifying each of the 11 R-genes known to confer resistance to late blight in potato. Our results indicate
that Rber is a new resistance gene, different from those recognized in S. demissum, and therefore, it has been named R
Pi-ber
according to the current system of nomenclature. In order to add new molecular markers around R
Pi-ber
, we used a PCR-based mapping technique, named MASP-map, which located R
Pi-ber
in a 3.9 cM interval between markers CT240 and TG63 on potato chromosome X. The location of R
Pi-ber
coincides with an area involved in resistance to different pathogens of potato and tomato. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|