Early postnatal development of the immune system in piglets: the redistribution of T lymphocyte subsets |
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Authors: | Stepanova H Samankova P Leva L Sinkora J Faldyna M |
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Institution: | a Department of Immunology, Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic b Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic c Dako Czech Republic, Brno, Czech Republic |
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Abstract: | In this study, we have characterised postnatal changes in T lymphocyte subsets, especially γδ T lymphocytes, in blood, spleen and lymph nodes. Detection was carried out using two-colour flow cytometry and three-colour immunohistochemistry. During ontogeny, there was a significant increase in the total percentage of γδ T cells in the spleen and blood. In the lymph nodes, there were no age-dependent changes in the total percentage of γδ T cells, but the percentage of the γδTCR+CD8+ subpopulation significantly increased. The tissue distribution of γδTCR+CD8+ and γδTCR+CD8− cells in the lymph nodes is random and not collocated with a particular area of the organ. Furthermore, postnatal development was characterised by an increasing frequency of CD8+CD3+CD4−γδTCR−, which was compensated by a decreasing proportion of CD4+ lymphocytes. Double positive CD4+CD8+ lymphocytes were rare during the first month of life and a significant age-dependent increase of these cells was found in all the compartments monitored. |
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Keywords: | Pig Ontogeny γδ T lymphocytes Flow cytometry Immunohistochemistry |
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