Osteosarcoma cell line growth inhibition by zoledronate-stimulated effector cells |
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Authors: | Muraro M Mereuta O M Carraro F Madon E Fagioli F |
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Institution: | Stem Cell Transplantation and Cellular Therapy Unit, Regina Margherita Children’s Hospital, Piazza Polonia 94, 10126 Turin, Italy |
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Abstract: | Bisphosphonates have a profound effect on bone resorption and are widely used in the treatment of osteoclast-mediated bone diseases. Zoledronic acid (ZA), a third-generation biphosphonate, has a potent antitumor activity and expands gammadelta (γδ) T cells endowed of major histocompatibility complex-unrestricted lytic activity. Many solid tumors express tumor-specific antigens on their surface, representing targets for immune effector T cells. Nevertheless, the immune surveillance against clinically manifested tumors is relatively inefficient. Therefore, we investigated the hitherto unknown effects of ZA activated γδ T cells of normal donors on osteosarcoma cell lines. γδ T cells were stimulated with ZA and low doses of interleukin-2, and then analyzed for proliferation and generation of effector activity against osteosarcoma cell lines. Our results show the potent anti-tumor activity of ZA-stimulated γδ T cells and the enhanced immunosensitivity of osteosarcoma cell lines to γδ T cells suggesting that osteosarcoma is another γδ T cell susceptible tumor type. |
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Keywords: | Gammadelta T cells Osteosarcoma Cytotoxicity |
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