Dual regulation of the T-type Ca(2+) current by serum albumin and beta-estradiol in mammalian spermatogenic cells |
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Authors: | Espinosa F López-González I Muñoz-Garay C Felix R De la Vega-Beltrán J L Kopf G S Visconti P E Darszon A |
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Affiliation: | Department of Genetics and Molecular Physiology, Institute of Biotechnology, UNAM, Avenida Universidad 2001, Cuernavaca, Mexico. |
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Abstract: | This study provides evidence for a novel mechanism of voltage-gated Ca(2+) channel regulation in mammalian spermatogenic cells by two agents that affect sperm capacitation and the acrosome reaction (AR). Patch-clamp experiments demonstrated that serum albumin induced an increase in Ca(2+) T current density in a concentration-dependent manner, and significant shifts in the voltage dependence of both steady-state activation and inactivation of the channels. These actions were not related to the ability of albumin to remove cholesterol from the membrane. In contrast, beta-estradiol significantly inhibited Ca(2+) channel activity in a concentration-dependent and essentially voltage-independent fashion. In mature sperm this dual regulation may influence capacitation and/or the AR. |
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