The basic nuclear proteins of the cellular slime mold Dictyostelium discoideum |
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| Authors: | M B Coukell I O Walker |
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| Affiliation: | 1. MOE Key Laboratory for Cellular Dynamics & Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China;2. Anhui Key Laboratory of Cellular Dynamics and Chemical Biology & Hefei National Research Center for Interdisciplinary Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, China;1. Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT, United States;2. Department of Life and Environmental Sciences, Faculty of Bioresource Sciences, Prefectural University of Hiroshima, Shobara, Hiroshima, Japan;3. Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, Kasuga, Tokyo, Japan;1. Department of Biology, Emory University, 1510 Clifton Road NE, Atlanta, GA 30322, USA;2. Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA;1. Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain;2. Center for Genomic Regulation, Doctor Aiguader 88, 08003 Barcelona, Spain;3. Universitat Pompeu Fabra (UPF), Doctor Aiguader 88, 08003 Barcelona, Spain;4. Centro Andaluz de Biología del Desarrollo (CABD), CSIC-Universidad Pablo de Olavide-Junta de Andalucía, Carretera de Utrera Km1, 41013 Sevilla, Spain;5. Institució Catalana de Recerca i Estudis Avançats, Pg Lluis Companys 23, 08010 Barcelona, Spain;6. Departament de Genètica, Universitat de Barcelona, 08028 Barcelona, Spain;1. LBE, Univ Montpellier, INRA, 102 avenue des Etangs, 11100 Narbonne, France;2. Suez, CIRSEE, 38 rue du Président Wilson, 78230 Le Pecq, France;1. Department of Animal Sciences, The Ohio State University, Columbus, OH, USA;2. The Ohio State University Interdisciplinary Human Nutrition Program, The Ohio State University, Columbus 43210, USA |
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| Abstract: | A procedure has been developed for the isolation of large numbers of nuclei from the cellular slime mold Dictyostelium discoideum. Nuclei isolated by this method contain DNA, RNA and protein in a ratio of 1:3.4:22. Basic proteins, equal in weight to the nuclear DNA, can be extracted from the nuclei with 1 M CaCl2. On urea polyacrylamide gels these basic proteins run as 6 fractions, 5 of which roughly co-migrate with calf thymus histones. On sodium dodecyl sulfate gels only 2 of 5 bands co-migrate with thymus histones. The histones of D. discoideum were compared electrophoretically with the histones of the acellular slime mold Physarum polycephalum and the cilliated protozoan Tetrahymena pyriformis. |
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