Effects of cryopreservation on the immunogenicity of porcine arterial allografts in early stages of transplant vasculopathy |
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Authors: | Solanes Núria Rigol Montserrat Khabiri Ebrahim Castellà Manuel Ramírez José Roqué Mercè Agustí Elba Roig Eulàlia Pérez-Villa Fèlix Segalés Joaquim Pomar José Luís Engel Pablo Massaguer Anna Martorell Jaume Rodríguez Jose Antonio Sanz Ginés Heras Magda |
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Institution: | Institut de Malalties Cardiovasculars, IDIBAPS (Institut d'Investigacions Biomèdiques Agustí Pi Sunyer), Barcelona, Spain. nsolanes@clinic.ub.es |
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Abstract: | BACKGROUND: The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS: 71 pigs (40 recipients and 31 donors) were used. Two femoral grafts per recipient animal were implanted for 3, 7, and 30 days. Types of grafts: fresh autograft as a control graft (n=19), fresh allograft (n=31) and cryopreserved allograft (n=30). Histological and immunohistochemical studies were performed. RESULTS: Fresh allografts compared to autografts showed intimal inflammatory infiltration at 3 days (328 vs. 0 macrophages/mm2; P<0.05) and 7 days (962 vs. 139 T lymphocytes/mm2; P<0.05) post-transplantation. At 30 days, there was a loss of endothelial cells, presence of luminal thrombus and aneurismal lesions (total area=15.8 vs. 8.4 mm2; P<0.05). Cryopreservation did not reduce these lesions nor modify endothelial nitric oxide synthase (eNOS) expression nor modify the number of animals that developed anti-SLA antibodies. Moreover, at 7 days, cryopreserved allografts compared to fresh allografts showed a higher expression of P-selectin (5 out of 5 vs. 1 out of 5; P<0.05) and, at 30 days, a greater inflammatory reactivity (2692 vs. 1107 T lymphocytes/mm2 in media; P<0.05) with a trend towards a higher presence of multinucleated giant cells than in the fresh ones. CONCLUSIONS: The cryopreservation method used maintained immunogenicity of allografts and increased the inflammatory reactivity found in fresh allografts up to 30 days of vascular transplantation. |
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Keywords: | Cryopreservation Vascular grafts Rejection Transplant vasculophathy |
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