| 放射性肺损伤细胞渗出及SP-A表达的实验研究 |
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| 引用本文： | 刘丹,赵玉霞,赵娜,门桐林,陈延治,姚雷.放射性肺损伤细胞渗出及SP-A表达的实验研究[J].中国组织化学与细胞化学杂志,2009,18(1). |
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| 作者姓名： | 刘丹 赵玉霞 赵娜 门桐林 陈延治 姚雷 |
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| 作者单位： | 中国医科大学附属第一医院肿瘤放射治疗科,沈阳,110001 |
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| 摘 要： | 目的探讨急性放射性肺损伤不同时间点病理和肺泡灌洗液与肺泡表面活性蛋白A(SP-A)表达的变化。方法健康雄性Wistar大鼠40只随机分为对照组(C组)和照射组(R组)。行6MV-X线全胸野照射,剂量率2Gy/min,单次剂量15Gy,源皮距1m,照射面积4.5cm×4.5cm。于照射后第1,2,4,8周取肺组织作HE、Masson染色,肺泡灌洗液进行细胞计数,蛋白免疫印迹(Westernblot)检测肺组织中SP-A蛋白表达。结果HE和Masson染色提示照射后的第1周始肺泡腔有炎性细胞渗出,继之间质水肿,第4及8周出现肺泡腔变小甚至结构破坏,局部实变,肺间质出现胶原纤维;肺泡灌洗液的细胞总数照射组与对照组相比各时间段都明显增高(P<0.01);照射组各时间点SP-A蛋白表达均明显下降(P<0.001),以第1、8周下降最为明显,第2、4周出现回升。结论SP-A蛋白参与放射性肺损伤的发生、发展过程,为进一步探讨放射性肺损伤的发病机制提供了实验依据。
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| 关 键 词： | 放射性肺损伤 支气管肺泡灌洗液 肺泡表面活性蛋白A |
EXPERIMENTAL STUDY ON CELL EXUDATION AND SP-A EXPRESSION IN RADIATION-INDUCED LUNG INJURY |
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| Liu Dan,Zhao Yuxia,Zhao Na,Men Tonglin,ChenYanzhi,Yao Lei.EXPERIMENTAL STUDY ON CELL EXUDATION AND SP-A EXPRESSION IN RADIATION-INDUCED LUNG INJURY[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). |
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| Authors: | Liu Dan Zhao Yuxia Zhao Na Men Tonglin ChenYanzhi Yao Lei |
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| Institution: | Department of Radiation Oncology;The First Affiliated Hospital;China Medical University;Shenyang 110001;China |
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| Abstract: | Objective To investigate the changes in pathology and expression of pulmonary surfatcant-associated protein A (SP-A) at different time points of acute radiation-induced lung injury.Methods Forty Wistar rats were randomly divided into two groups with 20 rats each: cont rol group (C) and radiation group (R). Rats in R group were thoracically radiated with linear accelaretor at a dose of 15Gy and a dose rate of 2Gy/min at the distance of 1m and in an area of 4.5 cm ×4.5 cm. HE and Masson staining of the rat lu... |
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| Keywords: | Radiation induced lung injury Bronchoalveolar lavage fluid Pulmonary surfatcant-associated protein A（SP-A） |
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