| 基因枪轰击成熟花粉粒转化玉米的研究 |
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| 引用本文： | 刘丹梅,杨君.基因枪轰击成熟花粉粒转化玉米的研究[J].生物技术通讯,2005,16(4):399-401. |
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| 作者姓名： | 刘丹梅 杨君 |
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| 作者单位： | 1. 辽东学院生物系,辽宁,丹东,118003
2. 大连理工大学,生物科学与工程系,辽宁,大连,116024 |
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| 摘 要： | 利用基因枪轰击花粉粒再授粉的基因转化途径，将豇豆胰蛋白酶抑制剂基因（CpTI）成功导入玉米受体中。经卡那霉素筛选结果表明，非转化植株经1000ppm卡那霉素溶液处理后白化、死亡，余下大量健壮、可育的抗性植株，转化率约1．59％。通过对抗性植株进行PCR和PCR—Southern检测，初步确定CpTI基因已导入玉米基因组。饲虫实验结果表明转化植株具有较强的抗虫性。
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| 关 键 词： | 基因枪 花粉粒 转化 玉米 |
| 文章编号： | 1009-0002(2005)04-0399-03 |
| 收稿时间： | 12 20 2004 12:00AM |
| 修稿时间： | 2004年12月20 |
Studies on Transforming CpTI Gene into Maize by Biolistics Bombardment of Pollen |
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| LIU Dan-mei,YANG Jun.Studies on Transforming CpTI Gene into Maize by Biolistics Bombardment of Pollen[J].Letters in Biotechnology,2005,16(4):399-401. |
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| Authors: | LIU Dan-mei YANG Jun |
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| Abstract: | The CpTI gene has been delivered into maize through pollen tube pathway by biolistics bombardment of pollen. These plants were carried on further screen of kanamycin and molecular test. Some of them were confirmed to be transgenic plants by assays of PCR and PCR-Southern. The transgenic maize has a certain degree of resistance to corn borer. |
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| Keywords: | biolisties bombardment pollen transformation maize |
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