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香菇半亲和双单杂交研究初报
引用本文:叶明,王岚岚,叶生梅,马桂荣,潘迎捷.香菇半亲和双单杂交研究初报[J].生物学杂志,2003,20(1):20-21,24.
作者姓名:叶明  王岚岚  叶生梅  马桂荣  潘迎捷
作者单位:1. 安徽工程科技学院生物化工系,芜湖,241000
2. 山东大学微生物技术国家重点实验室,济南,250100
3. 上海食用菌研究所食用菌遗传育种农业部部级重点实验室,上海,201106
基金项目:安徽省教育厅科研基金 (2 0 0 0j1 0 2 9),安徽省自然科学基金 (1 0 1 4 1 1 1 2 )资助
摘    要:选用一栽培株苏香两种孢子单核体自交后代作双核体亲本,与相应的孢子单核体进行半亲和双单杂交,得到6个杂交后代,均具有结实能力,说明半亲和双单杂交后代可以用于育种研究。大多数杂交菌株(核基因相同)在菌丝生长速率与CMC酶活方面显示出不同程度遗传差异,其中3号与4号菌株在菌丝生长速率与CMC酶活方面呈现明显杂种优势。

关 键 词:香菇  半亲杂交  双单杂交  液体出菇  菌丝生长速率  CMC酶活  杂种优势
文章编号:1008-9632(2003)01-0020-02

A preliminary study of hemi-compatible di-mon mating in lentinula edodes
YE Ming,WANG Lan-lan,YE Sheng-mei,MA Gui-rong,PAN Ying-jie.A preliminary study of hemi-compatible di-mon mating in lentinula edodes[J].Journal of Biology,2003,20(1):20-21,24.
Authors:YE Ming  WANG Lan-lan  YE Sheng-mei  MA Gui-rong  PAN Ying-jie
Institution:YE Ming1,WANG Lan-lan1,YE Sheng-mei1,MA Gui-rong2,PAN Ying-jie3
Abstract:The selfed progenies of sporualated monokaryons of a cultured strain in Lentinula edodes and their responding sporualated monokaryons were selected for hemi-compatiable di-mon mating.Six hybrids were obtained from their culture,which generated primordia in a liquid medium. Most hybrids of the same genome indicated the different genetic differences in the growth rate of mycelium and CMC,and No.4 of which showed obvious hybrid vigour.
Keywords:Lentinula edodes  hemi-compatiable di-mon mating  primordia formation in a liquid medium  growth rate of mycelium  CMC enzyme activity  hybrid vigour
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