Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR |
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| Authors: | Heller Loree C Davis Carisa R Peak K Kealy Wingfield David Cannons Andrew C Amuso Philip T Cattani Jacqueline |
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| Affiliation: | Esoteric Testing, Department of Pathology, Tampa General Hospital, PO Box 1289, Tampa, FL 33101, USA. lheller@tgh.org |
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| Abstract: | In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. |
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