| 人乳头瘤病毒(HPV)基因芯片的研究 |
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| 引用本文： | 刘翠华,马文丽,张宝,石嵘,郑文岭.人乳头瘤病毒(HPV)基因芯片的研究[J].微生物学报,2003,43(5):613-618,T001. |
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| 作者姓名： | 刘翠华 马文丽 张宝 石嵘 郑文岭 |
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| 作者单位： | 1. 第一军医大学分子生物学研究所,广州,510515
2. 广州军区广州总医院分子肿瘤学研究所,广州,510010 |
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| 基金项目： | 国家自然科学基金 (3 9880 0 3 2 )，广州市重点科技攻关项目 (99 Z 0 2 2 0 1)~~ |
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| 摘 要： | 探讨将基因芯片与限制性显示技术相结合对HPV进行基因检测和分型的方法。分离HPV6，11，16和18型的基因片段作为探针，纯化后应用PixSys 5500点样仪将其打印在氨基包被的玻片上制作HPV基因芯片，对HPV样品进行荧光标记后与芯片杂交，经清洗和干燥后对芯片进行扫描和结果分析。对HPV基因检测芯片的制作与检测的实验条件进行了初步研究，并对应用HPV基因芯片进行分型做了初步探讨。建立的检测芯片实验方法可行，并且显示了在HPV分型中的应用前景。
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| 关 键 词： | 基因芯片，限制性显示技术，人乳头瘤病毒 |
| 文章编号： | 0001-6209(2003)05-0613-06 |
Study on Development of DNA Microarrays for Human Papillomavirus (HPV) Diagnosis |
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| Liu Cuihua Ma Wenli,Zhang Bao Shi Rong Zheng Wenling.Study on Development of DNA Microarrays for Human Papillomavirus (HPV) Diagnosis[J].Acta Microbiologica Sinica,2003,43(5):613-618,T001. |
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| Authors: | Liu Cuihua Ma Wenli Zhang Bao Shi Rong Zheng Wenling |
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| Institution: | Institute of Molecular Biology, First Medical University, Guangzhou 510515, China. liuch@fimmu.edu.cn |
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| Abstract: | To study the technology for establishing DNA microarrays for the diagnosis of HP V.HPV6,11,16 and 18 gene fragments were isolated and printed onto aminosilane-c oated glass slides by PixSys 5500 microarray printer as probes to prepare the HP V. HPV samples, after labeled with Cy3 or Cy5, were hybridaized with the microar ray followed by scanning for analysis.The experimental condition for preparing t he HPV gene chips was investigated and the possibility of HPV genotying using DN A microarrays was discussed. The technique established in this study for prepari ng HPV DNA microarrays is applicable and has potential clinical application sign ificance. |
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| Keywords: | DNA microarrays Restriction display technology Human papillomavirus |
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