Changes of membrane fluidity in chemotactic peptide-stimulated polymorphonuclear leukocytes |
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| Authors: | M Valentino M Governa R Fiorini G Curatola |
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| Affiliation: | 1. Occupational Medicine Institute-University of Ancona, 60020 Torrette, ITALY;1. The School of Chemistry & Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China;2. School of Chemistry and Chemical Engineering, Liaoning Normal University, 850 Huanghe Road, Dalian, 116029, China;1. The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;3. Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142;4. Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139;5. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142;6. Howard Hughes Medical Institute, Cambridge, Massachusetts 02142;3. Department of Biochemistry and Molecular Biology;4. Complex Carbohydrate Research Center;6. Department of Computer Science, University of Georgia, Athens, Georgia 30602;5. Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104 |
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| Abstract: | Although the phenomenon of stimulus-response coupling in polymorphonuclear leukocytes involves a series of membrane events the influence of stimulation on membrane fluidity is to clarify. In our experiments we have used 1-(4-trimethylaminophenyl) 6-phenyl-1,3,5-hexatriene and 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization technique to evaluate membrane fluidity in living polymorphonuclear leukocytes after stimulation with N-formyl-methyonil-leucyl-phenylalanine peptide which has a well defined membrane receptor on the plasma membrane. We report that polymorphonuclear leukocytes stimulation increases 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene polarization, only when colcemid, a microtubule disrupting drug, is added to polymorphonuclear leukocytes. This can be viewed as an indirect evidence that microtubules are involved in the control of polymorphonuclear leukocytes membrane fluidity. On the contrary no changes have been observed with 1,6-diphenyl-1,3,5-hexatriene. This study indicates the potential use of 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene to evaluate the involvement of plasma membrane physical state during intact cell activity. |
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