Cloning of a gene coding for phosphotransacetylase fromEscherichia coli |
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Authors: | Hideko Yamamoto-Otake Asahi Matsuyama Eiichi Nakano |
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Affiliation: | (1) Research and Development Division, Kikkoman Corporation, 399 Noda, Noda-shi, Chiba, Japan |
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Abstract: | Summary A phosphotransacetylase gene (pta) has been cloned from a genomic DNA library ofEscherichia coli 1100, a derivative of strain K-12. The phosphotransacetylase activities ofpta+ plasmid-containing strains were amplified about 150-fold under control of thelac promoter. The molecular weight of the phosphotransacetylase was estimated to be about 81,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thepta gene was found to be downstream ofackA by a combination of restriction analysis and plasmid subcloning. It is located about 13 kb upstream of thepurF-folC-hisT region of the chromosome. |
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