Fish cell line (ULF-23HU) derived from the fin of the central mudminnow (Umbra limi): Suitable characteristics for clastogenicity assay |
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Authors: | Eun-Ho Park Jae-Seong Lee Ae-Kyng Yi Hisami Etoh |
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Institution: | (1) Department of Biology, College of Natural Sciences, Hanyang University, 133-791 Seoul, Korea;(2) Division of Biology, National Institute of Radiological Sciences, 260 Chiba, Japan |
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Abstract: | Summary A cell line (ULF-23HU) from the fin of the central mudminnow (Umbra limi) was characterized and tested for its suitability to assess cytogenetic damages induced by chemicals in fish. Cells of this
line exhibit a fibroblastlike appearance and grew optimal at 25°C in, TC-199 medium containing 10% fetal bovine serum, but
slower growth continued down to 4°C, where they could be stored for prolonged periods. Seeding efficiency of ULF-23HU cells
on the plastic substratum was approximately 85% in the above culture medium at 25°C. They had a 32-h cell cycle time taken
up by a 20-h S period as determined by the autoradiographic analysis of the fraction of labeled mitosis. Cultures showed relatively
high mitotic index (0.84 to 2.35%) during exponential growth phase lasting about 7 d. Karyological analysis of the cells at
the different subculture passages revealed constant chromosome modal number of 23 consisting of metacentric or submetacentric
chromosomes, which were primarily similar to those of in vivo cells, with one additional chromosome. The spontaneous sister
chromatid exchange rate was 5.3 per metaphse. When ULF-23HU cells were exposed toN-nitroso-N-methylurea, a clastogen in the mammalian cells, dose-dependent increases both in sister chromatid exchanges and chromosome
aberrations were clearly detected. These results on the growth kinetics and cytogenetic characteristics offered the high possibility
of the use of this cell line as a suitable in vitro model for clastogenicity studies in fish.
This work was supported by grants from Korea Sciences and Engineering Foundation and Japanese Government Research Awards for
Foreign Specialists to E.-H. Park. |
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Keywords: | Umbra limi cell line growth kinetics chromosome clastogenicity assay |
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