Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L. |
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Authors: | Katja Sesterhenn Melanie Distl Michael Wink |
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Affiliation: | 1.Institut für Pharmazie und Molekulare Biotechnologie,Universit?t Heidelberg,Heidelberg,Germany |
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Abstract: | Shoot, root, and callus cultures of Scrophularia nodosa L. (Scrophulariaceae) were established and cultivated in vitro. Iridoid glycosides, such as harpagoside, aucubin, and catalpol were identified by LC-ESI-MS and their contents determined by HPLC. For comparison intact plants of S. nodosa were analysed. In shoot cultures slightly lower amounts of detectable iridoid glycosides (4.36% dry weight) were determined than in the field grown plants (4.88%). Concentration of harpagoside was highest in leaves of field plants (1.05%) and in flowers of in vitro plantlets (1.10%). For aucubin the highest amount was found in the leaves of in vitro plantlets (1.67%) whereas the levels of aucubin in the leaves of field plants were remarkably lower. Catalpol was produced as a trace compound in intact plants and shoot cultures. Callus and root cultures were apparently not able to synthesise iridoid glycosides. |
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Keywords: | Scrophularia nodosa Iridoid glycosides Harpagoside Aucubin Plant in vitro culture |
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