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Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads |
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| Authors: | Wei-Peng Jiang Zhen Wang Li-Xin Xu Xin Peng Feng Chen |
| Institution: | *The Third Dental Center, Peking University School and Hospital of Stomatology, Beijing 100083, PR China;†Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, PR China;‡Department of Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, PR China |
| Abstract: | Saliva diagnostics utilizing nanotechnology and molecular technologies to detect oral squamous cell carcinoma (OSCC) has become an attractive field of study. However, no specific methods have been established. To refine the diagnostic power of saliva peptide fingerprints for the early detection of OSCC, we screened the expression spectrum of salivary peptides in 40 T1 stage OSCC patients (and healthy controls) using MALDI-TOF-MS combined with magnetic beads. Fifty proteins showed significantly different expression levels in the OSCC samples (P<0.05). Potential biomarkers were also predicted. The novel diagnostic proteomic model with m/z peaks of 1285.6 Da and 1432.2 Da are of certain value for early diagnosis of OSCC. |
| Keywords: | early diagnosis histatin-3 matrix-assisted laser-desorption ionization– time-of-flight– mass spectrometry (MALDI-TOF-MS) oral squamous cell carcinoma (OSCC) peptides saliva |