A dynein independent role of Tctex-1 at the kinetochore |
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Authors: | Chenshu Liu Jen-Zen Chuang Ching-Hwa Sung Yinghui Mao |
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Affiliation: | 1.Department of Pathology and Cell Biology; Columbia University College of Physicians and Surgeons; New York, NY USA;2.Department of Cell and Developmental Biology; Weill Medical College of Cornell University; New York, NY USA |
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Abstract: | Dynein light chains are accessory subunits of the cytoplasmic dynein complex, a minus-end directed microtubule motor. Here, we demonstrate that the dynein light chain Tctex-1 associates with unattached kinetochores and is essential for accurate chromosome segregation. Tctex-1 knockdown in cells does not affect the localization and function of dynein at the kinetochore, but produces a prolonged mitotic arrest with a few misaligned chromosomes, which are subsequently missegregated during anaphase. This function is independent of Tctex-1''s association with dynein. The kinetochore localization of Tctex-1 is independent of the ZW10-dynein pathway, but requires the Ndc80 complex. Thus, our findings reveal a dynein independent role of Tctex-1 at the kinetochore to enhance the stability of kinetochore-microtubule attachment. |
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Keywords: | Tctex-1 dynein mitosis kinetochore |
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