Thermal Motion of DNA in an MspA Pore |
| |
Authors: | Bo Lu Stephen Fleming Tamas Szalay Jene Golovchenko |
| |
Institution: | 1.Department of Physics, Harvard University, Cambridge, Massachusetts;2.School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts |
| |
Abstract: | We report on an experiment and calculations that determine the thermal motion of a voltage-clamped single-stranded DNA-NeutrAvidin complex in a Mycobacterium smegmatis porin A nanopore. The electric force and diffusion constant of DNA inside a Mycobacterium smegmatis porin A pore were determined to evaluate the thermal position fluctuations of DNA. We show that an out-of-equilibrium state returns to equilibrium so quickly that experiments usually measure a weighted average over the equilibrium position distribution. Averaging over the equilibrium position distribution is consistent with results of state-of-the-art nanopore sequencing experiments. It is shown how a reduction in thermal position fluctuations can be achieved by increasing the electrophoretic force used in nanopore sequencing devices. |
| |
Keywords: | |
|
|