Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli
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| Authors: | Zhaojuan Zheng Ying Xu Ye Sun Wending Mei Jia Ouyang |
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| Affiliation: | 1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People’s Republic of China.; 2. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, People’s Republic of China.; 3. Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing 210037, People’s Republic of China.; Imperial College London, UNITED KINGDOM, |
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| Abstract: | Trehalose is a non-reducing disaccharide, which can protect proteins, lipid membranes, and cells from desiccation, refrigeration, dehydration, and other harsh environments. Trehalose can be produced by different pathways and trehalose synthase pathway is a convenient, practical, and low-cost pathway for the industrial production of trehalose. In this study, 3 candidate treS genes were screened from genomic databases of Pseudomonas and expressed in Escherichia coli. One of them from P. stutzeri A1501 exhibited the best transformation ability from maltose into trehalose and the least byproduct. Thus, whole cells of this recombinant E. coli were used as biocatalyst for trehalose production. In order to improve the conversion rate of maltose to trehalose, optimization of the permeabilization and biotransformation were carried out. Under optimal conditions, 92.2 g/l trehalose was produced with a high productivity of 23.1 g/(l h). No increase of glucose was detected during the whole course. The biocatalytic process developed in this study might serve as a candidate for the large scale production of trehalose. |
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